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KMID : 1146920210510030327
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Journal of Pharmaceutical Investigation
2021 Volume.51 No. 3 p.327 ~ p.336
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Dose-dependent pro- or anti-scar-preventing responses of freeze-dried amniotic membrane extract in relation to suppression of connective tissue growth factor and α-smooth muscle actin
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Kang Mi-Rin
Lee Ae-Ri Cho
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Abstract
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Purpose: Dose-dependent pro- or anti-scar-preventing effects of amniotic membrane extract (AME) are unclear. This study investigated the dose-dependent effects of freeze-dried AME on connective tissue growth factor (CTGF) and α-smooth muscle action (α-SMA) (key determinants of excessive scarring) expression in an attempt to develop medication for scarless wound healing.
Methods: The inhibitory effect of freeze-dried AME on α-SMA was visualized through immunofluorescence confocal microscopy. The suppressive effect of 10 different concentrations of AME (0.0025?0.25 μg/mL) on CTGF and α-SMA was determined, and rates of collagen lattice contraction were measured.
Results: AME inhibited the trans-differentiation of fibroblasts to myofibroblasts, a hallmark of scar formation. AME downregulated CTGF and α-SMA in a dose-dependent manner; supplementation of 0.0175 μg/mL of AME in cell culture media downregulated CTGF (by 49%; p?
Conclusion: AME downregulates α-SMA and inhibits the trans-differentiation of fibroblasts to myofibroblasts in a dose-dependent manner. Treatment of cells with high AME concentrations retained high α-SMA concentration levels. A delicate balance between the wound healing properties and pro-fibrotic abilities of AME should be considered for selecting an appropriate therapeutic dose.
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KEYWORD
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Scar, Freeze-dried amniotic membrane extract, Connective tissue growth factor, α-Smooth muscle actin, Wound healing
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